Preparative thin-layer and column chromatography of prostaglandins
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چکیده
منابع مشابه
Preparative Thin-Layer (Planar) Chromatography
The various OPLC andRPC techniques are compared in Table 1. Study of the data shows that OPLC is an excellent technique for analytical separations and that RPC is more ideally suited as a preparative method for isolation of compounds from biological matrices. The advantage of combining online and ofSine separations and two-dimensional development can also be exploited in OPLC. The advantage of ...
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n Thin-layer chromatography (TLC) is being used increasingly for the separation of the lipid classes (1, 2) prior to the analysis of their fatty acids by gas-liquid chromatography (GLC). Malins and Mangold (3) have described the separation of the methyl esters of fatty acids of menhaden oil from other lipid constituents and stated “the gas chromatogram of the total methyl ester fraction agreed ...
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We have developed a high performance liquid chromatography (HPLC) method to separate lecithin from other phospholipid classes and to obtain lecithin from biologic materials. The separation was performed on a preparative 10-micron Spherisorb column with an optimized solvent system consisting of the following components: acetonitrile, isopropanol, methanol, water, and trifluoroacetic acid. The ad...
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Mixtures of lipids and phospholipids were separated by centrifugally accelerated thin-layer chromatography on a preparative scale (300-500 mg lipid mixture per run). The isolated lipids and phospholipids were identified by 1H and 13C NMR spectroscopy and their fatty acid composition was determined by GLC and GLC-MS of their methyl esters.
متن کاملThin-Layer (Planar) Chromatography
phy 665: 243. Junk GA, Richard JJ, Grieser MD et al. (1974) Use of Macroreticular resins in the analysis of water for trace organic contaminants. Journal of Chromatography 99: 745. Puig D and BarceloH D (1995) Off-line and on-line solidphase extraction followed by liquid chromatography for the determination of priority phenols in natural waters. Chromatographia 40: 435. Puig D and BarceloH D (1...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1969
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)43089-5